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Cellular Respiration Flashcards & Quiz

Glycolysis, the Krebs cycle and oxidative phosphorylation with full ATP accounting: flashcards and practice questions. Free, no sign-up.

Subject: biology

Summary

Cellular Respiration - ⚡ Cells break down glucose to release energy and capture it as ATP. ATP - 🔋 The molecule cells use to transfer energy for various cellular activities. Aerobic Respiration - 💨 Complete breakdown of glucose using oxygen, yielding much ATP. Anaerobic Respiration - 🚫 Partial breakdown of glucose without oxygen, yielding less ATP. Glycolysis - 🍎 Splits one glucose molecule into two pyruvate in the cytoplasm. Krebs Cycle - 🔄 Fully oxidizes acetyl-CoA in the mitochondrial matrix, producing carriers and ATP. Oxidative Phosphorylation - ✨ Produces most ATP using energy from electrons in the electron transport chain. Mitochondrion - 🧬 Organelle where most aerobic respiration stages occur, generating ATP. Electron Transport Chain - 🔗 Series of proteins in the inner mitochondrial membrane that pump protons. NAD⁺/NADH - ⚡ Coenzyme that picks up high-energy electrons and a proton to become NADH. Fermentation - 🧪 Regenerates NAD⁺ from NADH so glycolysis can continue without oxygen. Chemiosmosis - 💧 ATP synthesis driven by protons flowing through ATP synthase down their gradient. Oxygen Debt - 🏋️ Extra oxygen consumed after exercise to break down accumulated lactate. Final Electron Acceptor - 🎯 Oxygen's role at the end of the electron transport chain, forming water. Substrate-level Phosphorylation - ➡️ Making ATP by directly transferring a phosphate from an intermediate to ADP.

Flashcards

What is the primary purpose of cellular respiration?
Breaks down glucose to release energy as ATP.
What is the overall chemical equation for aerobic respiration?
C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O + energy
What is the typical ATP yield per glucose molecule in aerobic respiration?
About 30 to 32 ATP
What is the net ATP yield per glucose molecule from glycolysis in anaerobic respiration?
Net 2 ATP
What is the role of oxygen at the end of the electron transport chain?
Final electron acceptor
What happens to the electron transport chain and matrix stages if oxygen is absent?
Chain backs up, matrix stages stop.
What process regenerates NAD⁺ so glycolysis can continue without oxygen?
Fermentation
Which molecules deliver high-energy electrons to the electron transport chain?
NADH and FADH₂
What is pumped from the mitochondrial matrix into the intermembrane space by the electron transport chain?
Protons (H⁺)
Which enzyme drives the synthesis of ATP as protons flow back into the matrix?
ATP synthase
What is the mechanism called where ATP synthesis is driven by proton flow?
Chemiosmosis
Which stage of aerobic respiration produces the large majority of ATP?
Oxidative phosphorylation
What are the folds of the inner mitochondrial membrane called?
Cristae
What is the fluid interior of the mitochondrion that contains Krebs cycle enzymes?
Matrix
What does NAD⁺ become after picking up electrons and a proton?
NADH
What does FAD become after picking up electrons?
FADH₂
Why must NAD⁺ and FAD be regenerated after delivering electrons?
To be reused in earlier stages.
Where does glycolysis take place?
Cytoplasm
What is the ATP yield per glucose from the Krebs cycle, including the link reaction?
2 ATP
Where does oxidative phosphorylation occur?
Inner mitochondrial membrane
Which organisms perform lactic acid fermentation?
Human muscle cells, some bacteria
What are the products from pyruvate in lactic acid fermentation?
Lactate
Which organism performs alcoholic fermentation?
Yeast
What are the products from pyruvate in alcoholic fermentation?
Ethanol + CO₂
What is the definition of aerobic respiration?
Complete breakdown of glucose using oxygen.
What is the definition of anaerobic respiration?
Partial breakdown of glucose without oxygen.
What is the definition of glycolysis?
Splitting of glucose into two pyruvate.
What is pyruvate?
Three-carbon product of glycolysis.
What is acetyl-CoA?
Two-carbon fragment carrying carbon into Krebs cycle.
What is substrate-level phosphorylation?
Making ATP by direct phosphate transfer.
What is oxidative phosphorylation?
Making ATP using electron transport chain energy.
What is chemiosmosis?
ATP synthesis driven by proton flow.
What is the final electron acceptor?
Oxygen's role at the end of the chain.
What is the matrix in a mitochondrion?
Fluid interior where Krebs cycle occurs.
What are cristae?
Folds of the inner mitochondrial membrane.
What is oxygen debt?
Extra oxygen consumed after exercise.
What is the electron transport chain?
Protein complexes passing electrons and pumping protons.
What is ATP synthase?
Enzyme making ATP from proton flow.
What is the intermembrane space?
Compartment where protons accumulate.

Quiz questions

  1. What is the primary reason cells break down glucose gradually through a series of enzyme-catalyzed reactions in cellular respiration, rather than in a single step?

    Answer: To capture as much energy as possible in ATP, minimizing loss as heat.

    Cellular respiration is a controlled, stepwise process to maximize the capture of energy in ATP, preventing its dissipation as heat. This gradual release allows for efficient energy transfer.

  2. A cell is performing anaerobic respiration. What is the most significant consequence of the absence of oxygen for its ATP production compared to aerobic conditions?

    Answer: The cell will produce only a net 2 ATP per glucose, primarily because the electron transport chain cannot run.

    Without oxygen, the electron transport chain, which produces the vast majority of ATP (26-28 ATP), cannot operate. This leaves only glycolysis, yielding a net 2 ATP per glucose.

  3. How does the structure of the inner mitochondrial membrane contribute to the high ATP yield during oxidative phosphorylation?

    Answer: Its folds, called cristae, increase the surface area for the electron transport chain and ATP synthase.

    The inner mitochondrial membrane is folded into cristae, which significantly increases its surface area. This larger surface area accommodates more electron transport chain complexes and ATP synthase enzymes, facilitating a higher rate of oxidative phosphorylation and thus greater ATP production.

  4. Why is the regeneration of NAD⁺ from NADH critical for the continuous operation of glycolysis under both aerobic and anaerobic conditions?

    Answer: Glycolysis requires a steady supply of NAD⁺ to accept electrons and continue breaking down glucose.

    NAD⁺ acts as an electron carrier in glycolysis, picking up electrons to become NADH. If NAD⁺ is not regenerated, glycolysis would quickly deplete its supply of NAD⁺ and halt, stopping the breakdown of glucose and any ATP production from this pathway.

  5. What is the definition of 'aerobic respiration'?

    Answer: Complete breakdown of glucose using oxygen, yielding CO₂, water, and about 30+ ATP.

    Aerobic respiration is defined as the complete breakdown of glucose using oxygen, resulting in carbon dioxide, water, and a significant amount of ATP (around 30+).

  6. What is the definition of 'anaerobic respiration'?

    Answer: Partial breakdown of glucose without oxygen, yielding only the 2 ATP of glycolysis.

    Anaerobic respiration is characterized by the partial breakdown of glucose in the absence of oxygen, resulting in a much lower ATP yield (only the net 2 ATP from glycolysis).

  7. What is the definition of 'glycolysis'?

    Answer: Splitting of glucose into two pyruvate in the cytoplasm.

    Glycolysis is the initial stage of cellular respiration where one six-carbon glucose molecule is split into two three-carbon pyruvate molecules, occurring in the cytoplasm.

  8. What is 'pyruvate'?

    Answer: A three-carbon product of glycolysis.

    Pyruvate is the three-carbon molecule that results from the splitting of glucose during glycolysis.

  9. What is 'acetyl-CoA'?

    Answer: A two-carbon fragment, bound to coenzyme A, that carries carbon into the Krebs cycle.

    Acetyl-CoA is formed from pyruvate in the link reaction; it's a two-carbon acetyl group attached to coenzyme A, which then enters the Krebs cycle.

  10. What is 'substrate-level phosphorylation'?

    Answer: Making ATP by transferring a phosphate directly from an intermediate to ADP.

    Substrate-level phosphorylation is a direct method of ATP synthesis where a phosphate group is transferred from a high-energy intermediate molecule to ADP.

  11. What is 'oxidative phosphorylation'?

    Answer: Making ATP using energy from electrons passed down the electron transport chain.

    Oxidative phosphorylation is the process where ATP is synthesized using the energy released as electrons are passed along the electron transport chain, ultimately involving oxygen as the final acceptor.

  12. What is 'chemiosmosis'?

    Answer: ATP synthesis driven by protons flowing through ATP synthase down their gradient.

    Chemiosmosis is the specific mechanism within oxidative phosphorylation where the flow of protons (H⁺) down their electrochemical gradient through ATP synthase drives the synthesis of ATP.

  13. What is the 'final electron acceptor' in aerobic respiration?

    Answer: Oxygen

    At the end of the electron transport chain, oxygen accepts electrons and protons to form water. This is its crucial role in aerobic respiration.

  14. What is the 'matrix' in the context of cellular respiration?

    Answer: The fluid interior of the mitochondrion where the link reaction and Krebs cycle occur.

    The mitochondrial matrix is the fluid-filled space within the inner membrane where the link reaction and the Krebs cycle enzymes are located.

  15. What are 'cristae'?

    Answer: Folds of the inner mitochondrial membrane that increase surface area for the electron transport chain.

    Cristae are the characteristic folds of the inner mitochondrial membrane, which serve to greatly increase the surface area available for the electron transport chain and ATP synthase.

  16. What is 'oxygen debt'?

    Answer: The extra oxygen consumed after exercise to break down accumulated lactate.

    Oxygen debt refers to the increased oxygen intake after strenuous exercise, needed to metabolize the lactate produced during anaerobic respiration back into glucose or pyruvate.

  17. What is the 'electron transport chain'?

    Answer: A series of protein complexes in the inner membrane that pass electrons and pump protons.

    The electron transport chain is a crucial component of oxidative phosphorylation, located in the inner mitochondrial membrane, where electrons are passed along protein complexes to pump protons and generate a gradient for ATP synthesis.

  18. What is 'ATP synthase'?

    Answer: An enzyme that makes ATP as protons flow through it back into the matrix.

    ATP synthase is a key enzyme in chemiosmosis, utilizing the energy from the flow of protons down their electrochemical gradient to synthesize ATP from ADP and inorganic phosphate.

  19. What is the 'intermembrane space' in the context of cellular respiration?

    Answer: The compartment between the mitochondrial membranes where protons accumulate.

    The intermembrane space is the region between the outer and inner mitochondrial membranes where protons are pumped by the electron transport chain, creating the proton gradient.

  20. The process by which cells break down glucose to release energy and capture it in the form of ATP is called {0}.

    Answer: cellular respiration

    Cellular respiration is the fundamental process cells use to extract energy from glucose.

  21. Cells use {0} as the molecule to transfer energy from energy-releasing reactions to energy-requiring ones.

    Answer: ATP

    ATP (adenosine triphosphate) is the primary energy currency of the cell.

  22. The complete breakdown of glucose using oxygen, yielding CO₂, water, and about 30+ ATP, is known as {0}.

    Answer: aerobic respiration

    Aerobic respiration is characterized by its use of oxygen and high ATP yield.

  23. The partial breakdown of glucose without oxygen, yielding only the 2 ATP of glycolysis, is called {0}.

    Answer: anaerobic respiration

    Anaerobic respiration occurs in the absence of oxygen and produces much less ATP than aerobic respiration.

  24. {0} is the stage of cellular respiration that splits one six-carbon glucose into two three-carbon pyruvate molecules.

    Answer: Glycolysis

    Glycolysis is the initial stage of glucose breakdown, occurring in the cytoplasm.

  25. The fluid interior of the mitochondrion, called the {0}, contains the enzymes of the Krebs cycle.

    Answer: matrix

    The mitochondrial matrix is where the link reaction and Krebs cycle take place.

  26. {0} is the process of making ATP using energy from electrons passed down the electron transport chain.

    Answer: Oxidative phosphorylation

    Oxidative phosphorylation is the main ATP-producing stage in aerobic respiration.

  27. NADH and FADH₂ hand their electrons to the {0}, a series of protein complexes in the inner mitochondrial membrane.

    Answer: electron transport chain

    The electron transport chain is crucial for establishing the proton gradient used in ATP synthesis.

  28. ATP synthesis driven by protons flowing through ATP synthase down their gradient is known as {0}.

    Answer: chemiosmosis

    Chemiosmosis is the specific mechanism by which the proton gradient is used to generate ATP.

  29. {0} uses pyruvate to re-oxidize NADH back to NAD⁺ so glycolysis can keep running without oxygen.

    Answer: Fermentation

    Fermentation is essential for regenerating NAD⁺ in anaerobic conditions, allowing glycolysis to continue.

  30. {0} picks up electrons (with a proton) to become NADH, acting as an electron carrier.

    Answer: NAD⁺

    NAD⁺ is a key coenzyme that carries high-energy electrons during cellular respiration.

  31. The coenzyme {0} picks up electrons to become FADH₂, delivering them to the electron transport chain.

    Answer: FAD

    FAD is another important electron carrier, similar to NAD⁺.

  32. Most of aerobic respiration, including the Krebs cycle and electron transport chain, happens in the {0}.

    Answer: mitochondrion

    The mitochondrion is often called the 'powerhouse' of the cell due to its role in aerobic respiration.

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